Expected results and costs from OPU-ICSI vs classic ET

Efficiency of classic ET

  • One ovulation per cycle
  • 45% positive flushes
  • @14d: 80-85% pregnant
  • @45d: 70-75% pregnant
  • 4 embryos => 3 pregnant recipients
  • 45% positive flushes x 75% successful transfers
  • 33% of recipients are pregnant per flush
  • Average 1 foal per 3 flushes or 0,33 foals per flush

Estimated cost A.I with transfer of the embryo, excluded semen

  Recipient mare K.I. Hof Ter Leeuwe
Gynaecological follow up cycle fresh /frozen semen 125 /180€
Drugs ---
A.I. of the mare 30€
Embryoflush of the donor mare 200€
Transfer 1 embryo into recipient mare 200€
Recipient mare (lease) 3015
Total for 1 embryo after 1 cycle Semen not included minimum 3570€
On average 3 cycles needed for 1 foal Minimum 4680€

Efficiency of OPU-ICSI

  • Number of oocytes per OPU session
    • On average about 12+ oocytes are harvested per session
    • Number varies from 0 to 30+
  • Embryo production per OPU session
    • Average: >1 embryo (2,4) per OPU session
    • However: only 58% of all OPU sessions result in one or more embryo’s
  • Influence of the donor mare is also important
    • Mares that produce embryo(s) on first OPU/ICSI session: >>>70% chance to have embryo(s) on subsequent OPU attempts
    • Mares that do NOT produce an embryo on first OPU/ICSI session:>>>70% chance that they will not produce one on subsequent attempts
  • Stallion effect is minor compared to mare effect. Changing stallions often does not change the success rate
OPU

Pregnancy results with shipped oocytes and frozen ICSI embryo’s

  2018 2019 2020
@14d 72,2%
(n=56)
77%
(n=146)
74%
(n=186)
@45d 55,2% 66% 64%
losses 17% 11% 10%

Efficiency of OPU-ICSI

  • 2,4 embryos per OPU
  • 58% positive OPU’s
  • @14d: 72-78% pregnant
  • @45d: 60-66% pregnant
  • 4 embryos => 3 pregnant recipients
  • 58% positive OPU x 75% successful transfers x 2,4 embryo’s/OPU
  • 0,92 of recipients are pregnant per OPU
  • Average 1 foal per OPU

Estimated cost OPU-ICSI with transfer of the embryo, excluded semen

  Recipient mare K.I. Hof Ter Leeuwe Recipient mare client
Gynaecological exam before OPU ---€ ---€
OPU procedure with shipment to Avantea 1500€ 1500€
IVM & ICSI at Avantea (1 stallion for all oocytes) 380€ 380€
Additional stallion (semen preparation) 200€ 200€
Cryopreservation (per embryo) 420€ 420€
Thawing and transferring frozen ICSI embryo 400€ 400€
Recipient mare (lease) 3015€ 2015€
Total for 1 embryo in 1 OPU session Semen not included minimum 5715 minimum 4715