Efficiency of classic ET
- One ovulation per cycle
- 45% positive flushes
- @14d: 80-85% pregnant
- @45d: 70-75% pregnant
- 4 embryos => 3 pregnant recipients
- 45% positive flushes x 75% successful transfers
- 33% of recipients are pregnant per flush
- Average 1 foal per 3 flushes or 0,33 foals per flush
Estimated cost A.I with transfer of the embryo, excluded semen
| Recipient mare K.I. Hof Ter Leeuwe | |
|---|---|
| Gynaecological follow up cycle fresh /frozen semen | 125 /180€ |
| Drugs | --- |
| A.I. of the mare | 30€ |
| Embryoflush of the donor mare | 200€ |
| Transfer 1 embryo into recipient mare | 200€ |
| Recipient mare (lease) | 3015 |
| Total for 1 embryo after 1 cycle Semen not included | minimum 3570€ |
| On average 3 cycles needed for 1 foal | Minimum 4680€ |
Efficiency of OPU-ICSI
- Number of oocytes per OPU session
- On average about 12+ oocytes are harvested per session
- Number varies from 0 to 30+
- Embryo production per OPU session
- Average: >1 embryo (2,4) per OPU session
- However: only 58% of all OPU sessions result in one or more embryo’s
- Influence of the donor mare is also important
- Mares that produce embryo(s) on first OPU/ICSI session: >>>70% chance to have embryo(s) on subsequent OPU attempts
- Mares that do NOT produce an embryo on first OPU/ICSI session:>>>70% chance that they will not produce one on subsequent attempts
- Stallion effect is minor compared to mare effect. Changing stallions often does not change the success rate
Pregnancy results with shipped oocytes and frozen ICSI embryo’s
| 2018 | 2019 | 2020 | |
|---|---|---|---|
| @14d | 72,2% (n=56) |
77% (n=146) |
74% (n=186) |
| @45d | 55,2% | 66% | 64% |
| losses | 17% | 11% | 10% |
Efficiency of OPU-ICSI
- 2,4 embryos per OPU
- 58% positive OPU’s
- @14d: 72-78% pregnant
- @45d: 60-66% pregnant
- 4 embryos => 3 pregnant recipients
- 58% positive OPU x 75% successful transfers x 2,4 embryo’s/OPU
- 0,92 of recipients are pregnant per OPU
- Average 1 foal per OPU
Estimated cost OPU-ICSI with transfer of the embryo, excluded semen
| Recipient mare K.I. Hof Ter Leeuwe | Recipient mare client | |
|---|---|---|
| Gynaecological exam before OPU | ---€ | ---€ |
| OPU procedure with shipment to Avantea | 1500€ | 1500€ |
| IVM & ICSI at Avantea (1 stallion for all oocytes) | 380€ | 380€ |
| Additional stallion (semen preparation) | 200€ | 200€ |
| Cryopreservation (per embryo) | 420€ | 420€ |
| Thawing and transferring frozen ICSI embryo | 400€ | 400€ |
| Recipient mare (lease) | 3015€ | 2015€ |
| Total for 1 embryo in 1 OPU session Semen not included | minimum 5715 | minimum 4715 |